Phytotoxicity Testing with Higher Plants
Spirodela Duckweed Toxicity Tests
The SPIRODELA DUCKWEED TOXKIT contains all materials needed to preform 2 growth inhibition tests on Spirodela polyrhiza. Tests are preformed in 48 well microplates for a total of 5 test concentrations in 8 replicates.
The kit contains tubes with "turions" (vegetative buds) which can be stored and germinated on demand. No need for cultures.
Product Number TK63 SPIRODELA DUCKWEED TOXKIT
Resources:
Please review the step-by-step presentation on the SPIRODELA DUCKWEED growth inhibition test Procedure:
Duckweed Toxicity Test Presentation Click here to download the Spirodela duckweed Power Point. |
Comparison Data of MicroBioTests Click here for comparison data. |
Duckweed Toxicity Test Protocol Click here to download the Duckweed short protocol. |
Test criterion
- The Spirodela duckweed Toxkit is a 72 hour growth inhibition of the "first frond" after a 3 day exposure. Growth is determined by measurement of the area of the first frond at T=0 and T=72.
Reproducibility
- Turions of high quality are produced
in strictly controlled conditions. - High uniform quality of the growth and dilution media is acheived by dilutions in deionized water and Steinberg medium.
- Standard test containers constructed of biological inert materials ensure uniform exposure conditions.
- Quality Control Test with a reference chemical is described in detail allowing for accuracy and reproducibility.
Cost-Effectiveness
- The dromat "turions" can be germinated "on-demand", eliminating the need and the costs of continuous culturing and maintenance of stocks of test organisms.
- Minimal equipment required (laboratory incubator or temperature controlled room with illumination and conventional laboratory glassware).
- Shelf life of the turions is guaranteed for several months when stored correctly.
Contents
- Tubes with dormant turions, concentrated growth and dilution media, germination/test containers.
- Detailed Standard Operational Procedures, abbreviated Bench Protocol and Data treatment sheets.
- Specification sheets with batch numbers and 72 hour EC50 values for Quality Control Tests on the reference chemical (Potassium Chloride KCL).
User-Friendly
- Simple handlings and scorings.
- Total performance time of the assay approximately 3 hours.
- Area measurements of the first fronds are made on a photo of the multiwell test container, without any prior manipulation of the test organisms.
- Excel program for easy data treatment and calculations of the 72 hour EC50 can be obtained easily.
Sensitivity
- Comparable to the sensitivity of the Lemna duckweed species used in conventional duckweed assays.
Validation
- Duckweed assays are used in many laboratories globally for toxicity screening.
- The precision of the standardized Spirodela duckweed microbiotest has been determined in an extensive "International Interlaboratory Comparison" with participation of more than 50 laboratories from 20 countries.