Phytotoxicity Testing with Higher Plants

Phytotoxicity

phytotoxicity TestingKit TK61

The Phytotoxicity test kit is a 3 days toxicity test for phytotoxicity screening of soils, sludges, sediments, composts, effluents for irrigation, chemicals and biocides.

Each PHYTOTOXKIT contains all the materials necessary to perform one complete phytotoxicity test with one monocotyl and two dicotyl plant species, in 3 replicates. The assays are performed in special transparent test containers which allow for direct observations and length measurements by image analysis at the end of the test. Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of the very practical assays. Calibrated high quality seeds of the 3 selected test plants are included in the kits for the germination and early growth tests.

Product No. TK61 PHYTOTOXKIT

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Resources:

Please review the step-by-step presentation on the DAPHTOXKIT F Procedure:

TK61 - Phytotoxkit Test Presentation

phytotoxicity Testing Kit

Click here to download the Phytotoxkit Power Point.

EBPI Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here for comparison data.

Phytotest Kit Short Protocol

phytotoxicity TestingKit

Click here to downoad the latest version of the Phytotoxkit Short Protocol.

Test species

  • In accordance with conventional phytotoxicity assays the selected plant species encompass monocotyls as well as dicotyls.
  • The plants selected for the Phytotoxkit microbiotest are: the monocotyl Sorghum saccharatum (Sorgho) and the dicotyls Lepidium sativum (garden cress) and Sinapis alba (mustard).
  • The 3 former test species are frequently used in phytotoxicity analyses and have been selected for the Phytotoxkit microbiotest because of their very rapid germination and growth of roots and shoots, which allow observations and scoring after only 3 days.

Test criterion

  • Germination of the seeds and root (and wherever appropriate also shoot) growth of the 3 selected plant species on exposure to contaminated solid substrates or to chemicals, in comparison to germination and growth on a reference soil.

Reproducibility

  • The high quality seeds, the special standard test containers and the standard reference soil are a guarantee for the high repeatability and reproducibility of the assays, in comparison to conventional phytotoxicity assays.

User-friendly / Cost-Effective

  • Very rapid set up and scorings which allow handling multiple tests concurrently.
  • Direct observation of the germinated seeds and automatic measurement of the roots (and shoots) in the unique transparent test containers, by image analysis. A simple and convenient image analysis program can be provided free of charge on demand.
  • Vertical incubation of the flat test containers in their holders, requiring a minimum of shelf- and incubation space.
  •  “Image capturing” of the germinated seeds in the test containers with any type of “digital” equipment (camera, web camera or flatbed scanner).
  • Analyses and measurements can be deferred (since the “pictures” of the test plates are stored on computer) which is a major asset in comparison to conventional phytotoxicity tests.
  • Minimal equipment needed for test performance: small incubator - (webcam) camera or flatbed scanner, computer with image analysis program.

Contents

  • 18 transparent test containers with black filter paper, in 3 holders - 9 bags with reference soil - 3 tubes with seeds of the 3 test species.
  • Detailed Standard Operational Procedure brochure and abbreviated Bench Protocol.
  • Specification sheet with batch number of the seeds and the reference soil.

Sensitivity

  • The sensitivity of the Phytotoxkit has been compared with that of conventional phytotoxicity tests based on seed germination and early growth, and was found to give similar results. Additional validation studies are in progress.

Accessories package (Optional)

  • A package with various items for test preparation can be obtained separately. This package contains a 100 ml beaker, a 50 ml graduated cylinder, a 10 ml syringe, a 12 cm metal sieve (2 mm mesh), a spherical pestle and a flat pestle, a spatula and a pair of tweezers.

Optional Components for the Phytotoxkit

  • TK61     PHYTOTOXKIT with seeds of 3 higher plants (1 monocotyl & 2 dicotyls)

Test Biota

  • TB61     10 tubes with Sorgho seed (Sorghum saccharatum)
  • TB62     10 tubes with garden cress seed (Lepidium sativum)
  • TB63     10 tubes with mustard seed (Sinapis alba)

Test Containers and Test Items

  • TC61 6 holders with each 6 testplates and 6 black filter papers
  • TC62 120 testplates (bottom and lid) and black filter papers
  • TC63 300 testplates (bottom and lid) and black filter papers
  • TC64 600 testplates (bottom and lid) and black filter papers
  • TC65 10 holders for 6 testplates
  • TC66 20 holders for 6 testplates
  • TC67 50 holders for 6 testplates
  • TC71 120 black filter papers for testplates
  • TC72 300 black filter papers for testplates
  • TC73 600 black filter papers for testplates

Reference Soil

  • TM51 10 bags with reference OECD soil for Phytotoxkit tests
  • TM52 1 bag with 5 kg reference OECD soil for Phytotoxkit tests (and measuring cup)
  • TM53 1 bag with 10 kg reference OECD soil for Phytotoxkit tests (and measuring cup)

Equipment Needed (Optional)

  • Incubator with lateral illumination
  • Luxmeter
  • Digital Camera