Daphnia Toxicity Tests

Ceriodaphnia Toxicity Test

acute Ceriodaphtox ToxcityTest

The CERIODAPHTOXKIT F Toxicity Test Kit is a 24h toxicity test for screening of pure compounds, effluents, sediments, surface, ground and waste waters.

Like the Daphnia magna and Daphnia pulex the Ceriodaphnia toxicity testing kit contains all the materials necessary to perform six acute toxicity tests with the freshwater crustacean Ceriodaphnia dubia. The easy to follow instructions and detailed illustrations are provided in the toxicity kits for the conduct of range-finding and definitive tests. The test organisms (Ceriodaphnia dubia) are included in the kits as dormant eggs (ephippia) which can easily be hatched on demand in 80h, to supply the live biota for the conduct of the assays.




Please review the step-by-step presentation on the DAPHTOXKIT F Procedure:

 Daphnia Magna Test Presentation

acute Ceriodaphtox Kit

Click here to download the Daphnia magna Power Point.

EBPI Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here for comparison data. 

Ceriodaphnia Short Protocol

daphnia ToxicityTest 

Click here to download the latest version of the CERIODAPHTOXKIT F protocol.

Test Criterion

  • The CERIODAPHTOXKIT F toxicity test kit is a 24h assays based on mortality of the test organisms, with calculation of the 24h LC50.


  • Ephippia (dormant eggs) of high quality produced in strictly controlled conditions preclude variability associated with recruitment/maintenance of live stocks in conventional bioassays.
  • Highly uniform quality of the test medium is achieved by simple dilution of concentrated solutions of selected salts with deionized water.
  • Standardized micro plate test containers constructed of biologically inert materials ensure uniform exposure conditions.
  • A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.


  • Ephippia can be hatched "on demand", eliminating the need and the costs of continuous culturing and maintenance of test organisms.
  • Minimal equipment needed for test performance: light table or dissecting microscope - small incubator - conventional laboratory glassware.
  • Shelf-life of ephippia guaranteed for several months when stored properly, reducing test scheduling constraints.


  • Tubes with ephippia, concentrated hatching and toxicant dilution medium, hatching and test containers and micropipettes for the transfer of the organisms.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets and graphical LC50 calculation sheets.
  • Specification sheet with batch number of the ephippia and the media.


  • Simple handling and scoring.
  • Total performance time of the assay approximately 1 hour.
  • A floppy disc for easy Toxkit data treatment can be obtained on demand.

Optional Components for the CERIODAPHTOXKIT F

  • TK35      Acute CERIODAPHTOXKIT F with Ceriodaphnia dabia (6 tests per kit)

Test Biota

  • TB35     10 Tubes with Ceriodaphnia dabia ephippia

Test Containers

  • TC31     10 Multi-well plates for Cerodaphnia toxicity tests

Test Media

  • TM11    10 Sets of concentrated media for fresh water Daphnia pulex toxicity tests (for 10 X 1 EPA medium)

Test Items

  • TI12     10 Micropipettes forCerodaphnia tests
  • TI16     10 Microsieves for Daphnia tests
  • TI21     15 Small petri dishes (55mm)
  • TI27     10 Sheets Parafilm for rotifer and crustacean tests (3 X 2 squares)

Equipment Needed

  • Incubator with lateral illumination
  • Light table with traspartent stage for Daphnia tests
  • Luxmeter
  • Dissection microscope with transparent stage