Ames Tests

Ames 384-ISO™ Well Test

The Ames ISO procedure was developed to test mutagenic materials in a variety of sources like environmental air and water samples, foods, personal care products, and industrial effluents by the fluctuation assay which is a variation of the traditional “Ames Test”.

The ISO method provides researchers with a reliable test method which is facile to set up and run, amenable to samples in aqueous solution or suspension and produces an easily-detectable colorimetric endpoint after 2-3 days of incubation at 37 °C.

 

 

 

Product No: 6041 Ames 384-ISO Basic Kit

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Ames 384-ISO Basic Kit Includes:

• Reagents:
• 1 Ames Bacterial Strain, Growth Media and Control
• Reagents for 24 Test Points (NOTE: if S9 Activation is included, reagents for 48 Test Points are included)
• Sterile Distilled Water (100ml for Basic kit, 200ml for S9 Activation kits)

• Plastics:
• 2 24 well Plate (4 Plates if S9 Activation is included)
• 3 384 well Plates (6 Plates if S9 Activation is included)
• 15ml Tubes (2 units)
• Reaction Mixture bottle (1 unit)
• Ziploc Bags for incubation

TA100, TA98, TA1535, TA97a, WP2 uvrA, WP2 pkm101 (Please contact EBPI for request of different strains not listed)

 

 

 

 

 

 

Product No: 6041-2BS Ames 384-ISO Bacterial Strain Kit

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Ames 384-ISO Bacterial Strain Kit Includes:

• Reagents:
• 2 Ames Bacterial Strains, Growth Media and Controls
• Reagents for 48 Test Points (NOTE: if S9 Activation is included, reagents for 96 Test Points are included)
• Sterile Distilled Water (200ml for Basic kit, 400ml for S9 Activation kits)

• Plastics:
• 4 24 well Plate (8 Plates if S9 Activation is included)
• 6 384 well Plates (12 Plates if S9 Activation is included)
• 15ml Tubes (48 or 96)
• Reaction Mixture bottle (2 units)
• Ziploc Bags for incubation

This Ames test method is a modification of the Ames ISO fluctuation assay. Bacterial strains are pre-exposed to differing concentrations of the mutagenic compounds with a growth media in suspension for 100 minutes. Each dilution is run in triplicate.

EBPI has developed a line of Ames Test kits that are designed to meet OECD 471 Guidelines; these include the Ames 384-ISO (6041S9-OECD) as well as our highly popular Ames MOD-ISO (5041S9-OECD).  These OECD Ames Test kits allow users to run samples with and with a metabolic activation, in triplicates and 5 dilutions while still including all controls required (background, negative controls and positive controls).

TA100, TA98, TA1535, TA97a, WP2 uvrA, WP2 pkm101 (Please contact EBPI for request of different strains not listed)

 

Resources:

Please review EBPI's step-by-step presentations on the Muta-ChromoPlate Procedure:

Ames ISO 384 Well Presentation Click here to download EBPI's ISO Ames Test Power Point.

EBPI Ames Test Handout Click here to download EBPI's Muta-ChromoPlate Ames Test Handout.

Ames Test ISO Flow Chart Click here to download EBPI's latest version of the Ames ISO Test flow chart.

This method differs from the Muta-ChromoPlate method as the 384-well microplates used conserve reagents and plastic wastes. Laboratories knowledgeable with how to perform the Ames assay will enjoy the high performance of the ISO method with the added cost benefits and conservation of lab and incubation space which can be at a premium.

Bacterial Strains which can be included with the Ames ISO kit include:

TA100, TA98, TA1535, TA97a, WP2 uvr and WP2 uvr pKM101

S9 and Activation of Pro-Mutagens (Optional)

S9 is a crude liver enzyme extract that can, under certain conditions, convert materials without any genotoxic activity to active genotoxic entities. The chemical process involved may be different for different materials. In addition, the lifetime of the activated moieties is extremely variable: some may be extremely short-lived. This is the reason for incubating the S-9 with the bacteria and the tested material at the same time during the assays.

Ames Express Strains - A new alternate for the detection of Pro-Mutagens

EBPI is proud to officially announce the release of our NEW Ames bacterial strains, called the Ames-Express strains.  Ames-Express^TM strains are constructed from normal Ames bacterial strains (TA100, TA98, TA1535, TA97a, TA1538), but have been engineered to also express either human P450 or GST-theta liver enzymes internally, which promotes bioactivation of xenobiotic molecules into DNA reactive species in the absence of an S9 mix.   

The Ames Test ISO, Ames Modifed ISO and Muta-ChromoPlate Kit are designed to be run with the traditional Ames Test bacterial strains or EBPI's new Ames Express bacterial Strains.