Thamnocephalus platyurus Toxicity Tests

thamnocephalus Platyurus ToxicityTesting

24h Thamnocephalus platyurus toxicity test for screening of pure compounds, effluents, sediments, surface and ground waters, wastewaters, biotoxins and cyanotoxins

Each THAMNOTOXKIT F contains all the materials necessary to perform six acute toxicity tests with the freshwater crustacean Thamnocephalus platyurus. Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits as dormant eggs (cysts) which can easily be hatched on demand, in less than 24h, to supply the live biota for the conduct of the assays.




Please review the step-by-step presentation on the Thamnotoxkit Procedure:

Thamnocephalus Presentation


Click here to download the Thamnocephalus platyurus Power Point.

Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here for comparison data.

Thamnocephalus platyrus Protocol

thamnocephalus Platyurus ToxicityTest

Click here to download the latest version of the short Thamnotoxkit protocol.

Test criterion

  • The THAMNOTOXKIT F is a 24h assay based on mortality of the test organisms, with calculation of the 24hLC50.


  • Cysts of high quality produced in strictly controlled conditions preclude variability associated with recruitment/ maintenance of live stocks in conventional bioassays.
  • Highly uniform quality of the test medium is achieved by simple dilution of concentrated solutions of selected salts with de-ionized water.
  • Standardized micro-plate test containers constructed of biologically inert materials ensure uniform exposure conditions.
  • A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.


  • Cysts can be hatched on demand, eliminating the need and the costs of continuous culturing and maintenance of test organisms.
  • Minimal equipment needed for test performance: dissecting microscope – small incubator – conventional laboratory glassware.
  • Shelf-life of cysts guaranteed for several months when stored properly, reducing test scheduling constraints.


  • Tubes with cysts concentrated hatching and toxicant dilution medium, hatching and test ontainers and micropipettes for the transfer of the organisms.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets and graphical LC50 calculation sheets.
  • Specification sheet with batch number of the cysts and the media.


  • Simple handlings and scorings.
  • Total performance time of the assay approximately 1 hour.


  • Very sensitive assay.
  • In many cases more sensitive than other crustacean test species in acute assays.
  • Sensitive to biotoxins produced by blue-green algae.


  • Used in many laboratories worldwide for routine screening of chemicals and environmental samples.
  • Substantial number of publications reporting data for various uses.
  • Acute toxicity test with Thamnocephalus platyurus endorsed or taken into consideration for regulatory testing in several countries.

Optional Components for the THAMNOTOXKIT F

  • TK31     THAMNOTOXKIT F with Thamnocephalus platyurus (6 tests per kit)

Test Biota

  • TB31     10 Tubes with Thamnocephalus platyurus cysts

Test Containers

  • TC31     10 Multi-well plates for Thamnocephalus platyurus tests

Test Media

  • TM11     10 Sets of concentrated media for fresh water toxicity tests ( 10 X 1 EPA medium)

Test Items

  • TI11     10 Micropipettes for Thamnocephalus platyurus
  • TI21     15 Small petri dishes (55mm)
  • TI27     10 Sheets Parafilm for rotifer and crustacean tests (3 X 2 squares)

Equipment Needed

  • Incubator with lateral illumination
  • Light table with traspartent stage for Daphnia tests
  • Luxmeter
  • Dissection microscope with transparent stage