Ames Tests

Ames Modified ISO™ Test

This Ames test method is a modification of the Ames ISO fluctuation assay. Bacterial strains are pre-exposed to differing concentrations of the mutagenic compounds with a growth media in suspension for 100 minutes. Each dilution is run in triplicate.

The samples are then transferred, mixed with a reversion solution and plated onto a 96-well plate while using a 48-well evaluation format. The samples are incubated for 2-3 days and the number of revertant colonies (individual wells) are counted by colorimetric analysis.

 

 

 

Product No: 5041 Ames MOD-ISO Basic Kit

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Ames Modified ISO Basic Kit Includes:

• Reagents:
• 1 Ames Bacterial Strain, Growth Media and Control
• Reagents for 24 Test Points (NOTE: if S9 Activation is included, reagents for 48 Test Points are included)
• Sterile Distilled Water (200ml for Basic kit, 400ml for S9 Activation kits)

• Plastics:
• 12 96 well Plates (24 Plates if S9 Activation is included)
• 1 24 well Plate (2 Plates if S9 Activation is included)
• 15ml Tubes (24 or 48)
• Reaction Mixture bottle (1 unit)
• Ziploc Bags for incubation

TA100, TA98, TA1535, TA97a, WP2 uvrA, WP2 pkm101 (Please contact EBPI for request of different strains not listed)

 

 

 

 

 

 

Product No: 5041-2BS Ames MOD-ISO Bacterial Strain Kit

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Ames Modified ISO Bacterial Strain Kit Includes:

• Reagents:
• 2 Ames Bacterial Strains, Growth Media and Controls
• Reagents for 48 Test Points (NOTE: if S9 Activation is included, reagents for 96 Test Points are included)
• Sterile Distilled Water (200ml for Basic kit, 400ml for S9 Activation kits)

• Plastics:
• 4 24 well Plate (8 Plates if S9 Activation is included)
• 6 384 well Plates (12 Plates if S9 Activation is included)
• 15ml Tubes (48 or 96)
• Reaction Mixture bottle (2 units)
• Ziploc Bags for incubation

This Ames test method is a modification of the Ames ISO fluctuation assay. Bacterial strains are pre-exposed to differing concentrations of the mutagenic compounds with a growth media in suspension for 100 minutes. Each dilution is run in triplicate.

EBPI has developed a line of Ames Test kits that are designed to meet OECD 471 Guidelines; these include the Ames 384-ISO (6041S9-OECD) as well as our highly popular Ames MOD-ISO (5041S9-OECD).  These OECD Ames Test kits allow users to run samples with and with a metabolic activation, in triplicates and 5 dilutions while still including all controls required (background, negative controls and positive controls).

TA100, TA98, TA1535, TA97a, WP2 uvrA, WP2 pkm101 (Please contact EBPI for request of different strains not listed)

 

Resources:

Please review EBPI's step-by-step presentations on the Ames ISO Modification Test:

Ames Modified ISO Presentation Click here to download EBPI's Ames Modified ISO Power Point.

EBPI Ames Test Handout Click here to download EBPI's Ames Test Handout.

Ames Test Modified ISO Flow Chart Click here to download EBPI's latest version of the Ames Test Modified ISO flow chart.

This method combines the benefits of the ISO method while maintaining the ease-of-use of the Muta-ChromoPlate method. Smaller sample sizes are required and less reagents are used which is similar to the ISO, however, reversion media mixtures amounts and 96-well microplates facilitate pipetting and sample transfer to incubation step which mirrors the Muta-ChromoPlate Ames test procedure. The 48-well format reduces incubation plates required and plate covers reduce loss of highly volatile samples.

This method is a perfect hybrid between facility and efficiency and can be the preferred method depending on sample constituents.

S9 and Activation of Pro-Mutagens (Optional)

S9 is a crude liver enzyme extract that can, under certain conditions, convert materials without any genotoxic activity to active genotoxic entities. The chemical process involved may be different for different materials. In addition, the lifetime of the activated moieties is extremely variable: some may be extremely short-lived. This is the reason for incubating the S-9 with the bacteria and the tested material at the same time during the assays.

Ames Express Strains - A new alternate for the detection of Pro-Mutagens

EBPI is proud to officially announce the release of our NEW Ames bacterial strains, called the Ames-Express^TM strains.  Ames-Express^TM strains are constructed from normal Ames bacterial strains (TA100, TA98, TA1535, TA97a, TA1538), but have been engineered to also express either human P450 or GST-theta liver enzymes internally, which promotes bioactivation of xenobiotic molecules into DNA reactive species in the absence of an S9 mix.   

The Ames Test ISO, Ames Modifed ISO and Muta-ChromoPlate Kit are designed to be run with the traditional Ames Test bacterial strains or EBPI's new Ames Express bacterial Strains.