Thamnocephalus platyurus Toxicity Tests

thamnocephalus Platyurus Rapid ToxicityTesting

Thamnocephalus platyurus Rapid Toxicity Testing kit (30-60 min) for rapid detection of water contamination.

Each RAPIDTOXKIT contains all the materials necessary to perform 3 independent series of tests on up to 15 water samples each with larvae of the freshwater crustacean Thamnocephalus platyurus. Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of the very rapid assays. The test organisms are included in the kits as dormant eggs (cysts) which can easily be hatched on demand to supply the live biota for the conduct of the assays.




Please review the step-by-step presentation on the THAMNOTOXKIT Procedure:

Thamnocephalus Presentation

thamnocephalus RapidtoxkitPresentation

Click here to download the Thamnocephalus platyurus Power Point.

Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here for comparison data.


thamnocephalus Platyurus Rapid ToxicityTest TK37 

Click here to download the latest version of the Rapidtoxkit short protocol

Test criterion

  • The RAPIDTOXKIT is a 30-60 minutes sub lethal assay based on the decrease or the absence of ingestion of red microspheres by the test organisms exposed to contaminated waters.


  • Cysts of high quality produced in strictly controlled conditions preclude variability associated with recruitment/ maintenance of live stocks in conventional bioassays.
  • Highly uniform quality standard freshwater is included in the kits for the control tests in uncontaminated water.
  • Standardized hatching vessel and conical base test tubes constructed of biologically inert materials ensure uniform hatching and exposure conditions.


  • Cysts can be hatched on demand, eliminating the need and the costs of continuous culturing and maintenance of test organisms.
  • Minimal equipment needed for test performance: small incubator - stereo- microscope provided with strong incident illumination - 1 ml micropipette.
  • Shelf-life of cysts guaranteed for several months when stored properly, reducing test scheduling constraints.


  • Tubes with cysts, flask with hatching and control water medium, hatching vessels, conical base sub-sampling and test tubes in holders, vial with red microspheres, vial with fixative, observation plates with covers, and mirror with sliding white strip.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets.
  • Specification sheet with batch number of the cysts, standard freshwater medium, red microspheres and fixative.


  • Very simple handlings and scorings.
  • Total performance time of the assay from 30 to 90 minutes.


  • Very sensitive sub lethal assay.
  • More sensitive than bacterial luminescence inhibition tests for particular chemical compounds and biotoxins.
  • Detection threshold for toxicants is below Human Lethal Dose (HLD) which makes the Rapidtoxkit particularly suited for assessment of (accidental or deliberate) water contamination threats.

Supporting equipment

  • The “availability” of the live test organisms can be selected with the aid of the “Mini Toxkit Incubator” which allows programming the exact hatching time of the cysts up to 4 days in advance.
  • A low cost ring light with holder has been developed specifically for the Rapidtoxkitfor the observation of the test organisms under the stereomicroscope.

Optional Components for the THAMNOTOXKIT F

  • TK31     THAMNOTOXKIT F with Thamnocephalus platyurus (6 tests per kit)

Test Biota

  • TB31     10 Tubes with Thamnocephalus platyurus cysts

Test Containers

  • TC31     10 Multi-well plates for Thamnocephalus platyurus tests

Test Media

  • TM11     10 Sets of concentrated media for fresh water toxicity tests ( 10 X 1 EPA medium)

Test Items

  • TI11     10 Micropipettes for Thamnocephalus platyurus
  • TI21     15 Small petri dishes (55mm)
  • TI27     10 Sheets Parafilm for rotifer and crustacean tests (3 X 2 squares)

Equipment Needed

  • Incubator with lateral illumination
  • Light table with traspartent stage for Daphnia tests
  • Luxmeter
  • Dissection microscope with transparent stage