Rotifer Toxicity Tests

Brachionus calyciflorus Rotifer Toxicity Test

rotifer RotoxkitF ToxicityTesting

Rotifer Short Term Chronic 24h Toxicity Test for screening of pure compounds, effluents, sediments, surface, ground and waste waters

The Chronic ROTOXKIT F contains all the materials necessary to perform three short-chronic bioassays. The test species is the freshwater rotifer* Brachionus calyciflorus.

Rotifers are ecologically very important members of many aquatic communities. With copepod and cladoceran crustaceans they are the major constituents of freshwater zooplankton, with turnover rates which are higher than those of the crustaceans.  Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits as dormant eggs (cysts) which can easily be hatched on demand in less than 24h, to supply the live biota for the conduct of the assays.

Product No. TK23 ROTOXKIT F



Please review the step-by-step presentation on the ROTOXKIT F Procedure:

Brachionus calyciflorus Presentation

brachionusCalyciflorus Presentation

Click here to download the TK23 Rotifer Power Point.

EBPI Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here for comparison data.

TK23 Rotifer Toxicity Test Protocol

rotifer RotoxkitF ToxicityTest

Click here to download the latest version of the Chronic ROTOXKIT F short protocol.


  • The ACUTE ROTOXKIT F is a 24h assay based on mortality of the test organisms, with calculation of the 24hLC50.
  • The SHORT-CHRONIC ROTOXKIT F measures the decrease in reproduction of the rotifers under toxic stress after 48h exposure, with calculation of the 48h median growth inhibition (48hEC50). NOEC and LOEC values can be determined with specific data treatment program.


  • Cysts of high quality produced in strictly controlled conditions preclude variability associated with recruitment/ maintenance of live stocks in conventional bioassays.
  • Highly uniform quality of the test medium is achieved by simple dilution of concentrated solutions of selected salts with de-ionized water.
  • Standardized micro-plate test containers constructed of biologically inert materials ensures uniform exposure conditions.
  • A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.


  • Cysts can be hatched on demand, eliminating the need and the costs of continuous culturing and maintenance of test organisms.
  • Minimal equipment needed for test performance: dissecting microscope – small incubator – conventional laboratory glassware.
  • Shelf-life of cysts guaranteed for several months when stored properly, reducing test scheduling constraints.


  • Tubes with cysts, concentrated hatching and toxicant dilution medium, hatching/test containers and micropipettes for the transfer of the organisms.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets and graphical L (E) C50 calculation sheets.
  • For the short-chronic tests : tubes with live micro-algae immobilized in algal beads, a vial with matrix dissolving medium, tubes with a pre-feeding powder and a vial with Lugol fixative for easy counting of the rotifers.
  • Specification sheet with batch number of the cysts,   the media and the algal beads.


  • Specially designed « all in one » micro-plates with hatching trough, rinsing troughs and 36 (acute) or 48 (short-chronic) test wells for easy and rapid transfer of the test organisms under a dissecting microscope.
  • Total performance time approximately 1h for the acute assays and 2h for the short-chronic tests.
  • A floppy disc for easy Toxkit data treatment can be obtained on demand.


  • Very chemical dependent, but highly sensitive to particular chemicals and mixtures.
  • Growth inhibition NOEC’s determined with the short-chronic ROTOXKIT F are ecologically meaningful thresholds for the impact of toxicants on this important group of biota in aquatic food chains.


  • The acute toxicity test with Brachionus calyciflorus is an ASTM Standard since 1991.
  • A 48h growth inhibition test with rotifers (with Brachionus calyciflorus as test species) is proposed in the 20th Edition (1998) of Standard Methods for the Examination of Water and Wastewater; the short-chronic rotifer micro-biotest is also under consideration as a new AFNOR Guideline in France.

Optional Components for the Short-Chronici ROTOXKIT F

  • TK23     Short-Chronic ROTOXKIT F with Brachionus calyciflorus (3 tests per kit)

Test Biota

  • TB21     10 Tubes with Brachionus calyciflorus cysts

Test Containers

  • TC22     10 Multi-well plates for short-chronic rotifer toxicity tests

Test Media

  • TM11     10 Sets of concentrated media for fresh water toxicity tests ( 10 X 1 EPA medium)

Test Foods

  • TF11     5 Tubes with algal food for short-chronic rotifer tests + de-immobilization medium
  • TF15     10 Tubes with Roti-Rich for rotifer pre-feeding

Test Items

  • TI11     10 Micropipettes for rotifer tests
  • TI18     5 Vials with Lugol fixative for short-chronic rotifer tests
  • TI21     15 Small petri dishes (55mm)
  • TI27     10 Sheets of Parafilm for rotifer and crustacean tests (3 X 2 squares)

Equipment Needed

  • Incubator with lateral illumination
  • Light table with traspartent stage
  • Luxmeter
  • Dissection microscope with transparent stage