Rotifer Toxicity Tests

Marine Water Rotifer Toxicity Test

rotiferM rotoxkitM ToxicityTests

Marine Water 24 - 48h Rotifer Toxicity Test for screening of pure compounds, contaminated seawater and estuarine/marine sediments as well as effluents/wastes released in estuaries or coastal waters.

The ROTOXKIT M contains all the materials necessary to perform six acute toxicity tests with the marine rotifer* Brachionus plicatilis.  
Rotifers are ecologically important members of freshwater and marine aquatic communities and have very rapid turnover rates. 
Easy to follow instructions and detailed illustrations are provided in the toxicity kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits as dormant  eggs (cysts) which can easily be hatched on demand, in less than 24h, to supply the live biota for the conduct of the assays.

Product No TK22 ROTOXKIT M



Please review the step-by-step presentation on the DAPHTOXKIT F Procedure:

Brachionus calyciflorus Presentation

marine Water Rotifer Toxicity Test Kit

Click here to download the TK23 Rotifer Power Point.

EBPI Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here for comparison data.

TK23 Rotifer Toxicity Test Protocol

rotiferM rotoxkitM ToxicityTesting

Click here to download EBPI's latest version of the Chronic ROTOXKIT F short protocol.

Test criterion

  • The ROTOXKIT M is a 24-48h assay based on mortality of the test organisms, with calculation of the 24hLC50.


  • Cysts of high quality produced in strictly controlled conditions preclude variability associated with recruitment/ maintenance of live stocks in conventional bioassays.
  • Highly uniform quality of the test medium is achieved by simple dilution of concentrated solutions of selected salts with de-ionized water.
  • Standardized micro-plate test containers constructed of biologically inert materials ensure uniform exposure conditions.
  • A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.


  • Cysts can be hatched on demand, eliminating the need and the costs of continuous culturing and maintenance of test organisms.
  • Minimal equipment needed for test performance: dissecting microscope - small incubator - conventional laboratory glass-ware.
  • Shelf-life of cysts guaranteed for several months when stored properly, reducing test scheduling constraints.


  • Tubes with cysts, concentrated hatching and toxicant dilution medium, hatching/test containers and micropipettes for the transfer of the organisms.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets and graphical LC50 calculation sheets.
  • Specification sheet with batch number of the cysts and the media.


  • Specially designed « all in one » micro-plates with hatching trough, rinsing troughs and test wells for easy and rapid transfer of the test organisms under a dissecting microscope.
  • Simple handlings and scorings· 
  • Total performance time approximately 1 hour.


  • Chemical dependent, though sensitive to particular chemicals and mixtures.
  • Sensitivity can be increased substantially by extending the exposure time from 24h to 48h


  • Brachionus plicatilis is a euryhaline species which allows performing bioassays on waters in a salinity range from 5ppt (brackish water) to 35ppt (seawater). A list of selected references is available upon request.

Optional Components for the Acute ROTOXKIT M

  • TK22     Acute ROTOXKIT M with Brachionus plicatilis (6 tests per kit)

Test Biota

  • TB22     10 Tubes with Brachionus plicatilis cysts

Test Containers

  • TC21     10 Multi-well plates for acute rotifer tests

Test Media

  • TM12     10 Sets of concentrated media for marine/estuarine toxicity tests ( 10 X 1 ASPM medium)

Test Items

  • TI11     10 Micropipettes for rotifer tests
  • TI21     15 Small petri dishes (55mm)
  • TI27     10 Sheets Parafilm for rotifer and crustacean tests (3 X 2 squares)

Equipment Needed

  • Incubator with lateral illumination
  • Light table with traspartent stage
  • Luxmeter
  • Dissection microscope with transparent stage