Rotifer Toxicity Tests

24 hour Acute Rotifer Toxicity Test

rotifer RotoxkitF ToxicityTesting

The Rotifer Rotoxkit F is a 24h Acute Toxicity Test for screening of pure compounds, effluents, sediments, surface, ground and waste waters

The ACUTE ROTOXKIT F contains all the materials necessary to perform six acute toxicity tests. The test species is the freshwater rotifer* Brachionus calyciflorus.

Rotifers are ecologically very important members of many aquatic communities. With copepod and cladoceran crustaceans they are the major constituents of freshwater zooplankton, with turnover rates which are higher than those of the crustaceans. Easy to follow instructions and detailed illustrations are provided in the kits for the conduct of range-finding and definitive tests. The test organisms are included in the kits as dormant eggs (cysts) which can easily be hatched on demand, in less than 24h, to supply the live biota for the conduct of the assays.

Product No. TK21 Acute Rotoxkit F



Please review the step-by-step presentation on the DAPHTOXKIT F Procedure:

Brachionus calyciflorus Presentation

brachionusCalyciflorus Presentation

Click here to download the TK23 Rotifier Power Point.

Comparison Data of MicroBioTests

comparison Data MicroBiotests

Click here to download the TK23 Rotifier Power Point.

TK21 24hour Rotifier Toxicity Test Protocol

rotifer RotoxkitF ToxicityTest

Click here to download EBPI's latest version of the Acute ROTOXKIT F short protocol.


  • The ACUTE ROTOXKIT F is a 24h assay based on mortality of the test organisms, with calculation of the 24hLC50.
  • The SHORT-CHRONIC ROTOXKIT F measures the decrease in reproduction of the rotifers under toxic stress after 48h exposure, with calculation of the 48h median growth inhibition (48hEC50). NOEC and LOEC values can be determined with specific data treatment program.


  • Cysts of high quality produced in strictly controlled conditions preclude variability associated with recruitment/ maintenance of live stocks in conventional bioassays.
  • Highly uniform quality of the test medium is achieved by simple dilution of concentrated solutions of selected salts with de-ionized water.
  • Standardized micro-plate test containers constructed of biologically inert materials ensures uniform exposure conditions.
  • A Quality Control Test with a reference chemical is described in detail, for accuracy and reproducibility check.


  • Cysts can be hatched on demand, eliminating the need and the costs of continuous culturing and maintenance of test organisms.
  • Minimal equipment needed for test performance: dissecting microscope – small incubator – conventional laboratory glassware.
  • Shelf-life of cysts guaranteed for several months when stored properly, reducing test scheduling constraints.


  • Tubes with cysts, concentrated hatching and toxicant dilution medium, hatching/test containers and micropipettes for the transfer of the organisms.
  • Detailed Standard Operational Procedure brochure, abbreviated Bench Protocol, data scoring sheets and graphical L (E) C50 calculation sheets.
  • For the short-chronic tests: tubes with live micro-algae immobilized in algal beads, a vial with matrix dissolving medium, tubes with a pre-feeding powder and a vial with Lugol fixative for easy counting of the rotifers.
  • Specification sheet with batch number of the cysts, the media and the algal beads.


  • Specially designed all in one micro-plates with hatching trough, rinsing troughs and 36 (acute) or 48 (short-chronic) test wells for easy and rapid transfer of the test organisms under a dissecting microscope.
  • Total performance time approximately 1h for the acute assays and 2h for the short-chronic tests.


  • Very chemical dependent, but highly sensitive to particular chemicals and mixtures.
  • Growth inhibition NOEC’s determined with the short-chronic ROTOXKIT F are ecologically meaningful thresholds for the impact of toxicants on this important group of biota in aquatic food chains.


  • The acute toxicity test with Brachionus calyciflorus is an ASTM Standard since 1991.
  • A 48h growth inhibition test with rotifers (with Brachionus calyciflorus as test species) is proposed in the 20th Edition (1998) of Standard Methods for the Examination of Water and Wastewater; the short-chronic rotifer microbiotest is also under consideration as a new AFNOR Guideline in France.

Optional Components for the Acute ROTOXKIT F

  • TK21     Acute ROTOXKIT F with Brachionus calyciflorus (6 tests per kit)

Test Biota

  • TB21     10 Tubes with Brachionus calyciflorus cysts

Test Containers

  • TC21     10 Multi-well plates for acute rotifer tests

Test Media

  • TM11     10 Sets of concentrated media for fresh water toxicity tests ( 10 X 1 EPA medium)

Test Items

  • TI11     10 Micropipettes for rotifer tests
  • TI21     15 Small petri dishes (55mm)
  • TI27     10 Sheets Parafilm for rotifer and crustacean tests (3 X 2 squares)

Equipment Needed

  • Incubator with lateral illumination
  • Light table with traspartent stage for
  • Luxmeter
  • Dissection microscope with transparent stage